Methods

Cell Sorting: Cell sorting is a technique used to separate and collect specific cell populations from a heterogeneous mixture based on distinct characteristics like size, shape, or surface markers. This is commonly performed using flow cytometry or other automated systems to isolate specific cells for further analysis or experimentation.

Classical Flow Cytometry Analysis: Classical flow cytometry analysis involves measuring the physical and chemical properties of cells as they flow in a fluid stream through a laser beam. The scattered light and fluorescence emitted by labeled antibodies provide data on cell size, granularity, and specific markers, enabling the analysis of cell populations.

Spectral Cytometry: Spectral cytometry is an advanced technique that captures the full fluorescence spectrum emitted by multiple fluorochromes simultaneously. It allows for the analysis of more parameters in a single sample compared to traditional flow cytometry, improving the resolution of complex biological data.

Image Cytometry: Image cytometry combines traditional cytometry with microscopy, allowing for the visualization and quantification of cellular characteristics such as morphology, protein expression, and other parameters in individual cells. It captures high-resolution images for in-depth analysis, often using automated systems.

Multiplex Cytokine Analysis: Multiplex cytokine analysis is a technique used to measure multiple cytokines or biomarkers in a single sample. It employs techniques like Luminex or ELISA to quantify various proteins simultaneously, allowing for comprehensive analysis of immune responses and other biological processes.