Getting started
Antigen requirements
In general, we need 2 mg of purified (soluble) protein lacking any fusion partners commonly used to increase solubility (GST, MBP, SUMO). Please use Tris or HEPES based buffers and stick to our antigen formulation info sheet, which is provided on request. For phage display, the target protein will typically be immobilized on magnetic beads (biotinylated protein on streptavidin beads, Fc fusions on protein A beads, GST fusions on glutathione beads, MBP fusions on amylose beads). If already available, please provide target proteins appropriate for immobilization as well. If purified protein is not available, we can support you with information on expression strategies, cooperating protein production core facilities, and commercial vendors. For difficult-to-express targets such as multi-span transmembrane proteins we can discuss different immunization and selection strategies, including DNA immunization and cell-based panning.
Address
How to find us
Arrival by bus and train or map
From Bonn main station you can take the bus line 600 (direction Ippendorf Altenheim) and 601 (direction Venusberg) to the bus stop “Uniklinikum Nord, Uniklinikum Hauptpforte or Uniklinikum Süd”. Line 630 runs in the Bonn city area without stopping at the main station. Please use the night bus (N5) between midnight and 5:30 a.m.
The Core Facilities thank the German Research Foundation for continuous support.
