Instrumentation
ThermoFisher Orbitrap Tribrid Fusion Lumos
The „Lumos“ is our flagship: A modern hybrid instrument that has a quadrupole, Orbitrap, and ion-trap mass analyzer. In combination with three different fragmentation methods (including electron transfer dissociation – ETD) it is one of the most versatile instruments available. All high throughput projects benefit from its sensitivity and speed. Modern measurement modes are available: analyses of intact proteoforms, data-independent (DIA) and targeted techniques (PRM).
ThermoFisher Orbitrap Velos
The Orbitrap Velos has two detectors: a fast low resolution linear ion trap and a high resolution, high accuracy Orbitrap detector which can be used in parallel. This instrument is used for low complexity samples where high resolution in MS2 spectra is not needed.
nanocLC systems
Three ultra-high performance liquid chromatographs for nanoflow (~300 nl/min) are used for coupling with mass spectrometers. These systems perform chromatographic separations very reproducibly and assure a stable delivery of analytes to the mass spectrometers. Columns are made in-house.
A variety of sample preparation devices can be used to handle larger sets of samples. If necessary protein complexity is reduced by various fractionation methods.
Advion Triversa Nanomate
A Nanomate nanospray robot can be used for automated delivery of small sample amounts for direct infusion on the Orbitrap Velos spectrometer.
The Core Facilities thank the German Research Foundation for continuous support.
