Microscopy Core Facility

The Microscopy Core Facility provides all members of the university with access to a broad range of advanced light and electron microscopy technologies. Our services support a wide spectrum of imaging applications, from live-cell imaging and high-resolution analysis of tissue sections to ultrastructural studies using electron microscopy. In addition to access to state-of-the-art instruments, we offer expert support in experimental design, sample preparation, and image acquisition. Whether you're working with cells, tissues, or large biological samples, we are here to help you achieve the best possible imaging results for your research.

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Instruments

We provide high-end microscopes tailored for live cell imaging, fixed tissue sections, and large organ studies. Our equipment includes light and electron microscopes.

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Services

We offer scientific and technical support for experiment design, image acquisition, and analysis. For electron microscopy we also provide sample preparation services.

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Teaching

We offer individual training courses on our microscopes, as well as regular workshops to introduce you to microscopy in general or the basics of image analysis.

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FAQ

Answers to frequently asked questions about the PPMS booking system, the billing of core facility services and much more can be found in our FAQ section on Confluence.

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Consulting

Are you interested in our services? Please get in touch with us. We are happy to help!

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Acknowledgement

You would like to publish your results but do not know how to take the support of the Core Facility into account? Here you will find detailed instructions.

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170+

active users

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active groups

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instruments

News from the Core Facility / Science news

Bacterium Produces “Organic Dishwashing Liquid” to Degrade Oil

The marine bacterium Alcanivorax borkumensis feeds on oil, multiplying rapidly in the wake of oil spills, and thereby accelerating the elimination of the pollution, in many cases. It does this by producing an “organic dishwashing liquid” which it uses to attach itself to oil droplets. Researchers from the University of Bonn, RWTH Aachen University, Heinrich Heine University Düsseldorf and research center Forschungszentrum Jülich have now discovered the mechanism by which this “organic dishwashing liquid” is synthesized. Published in the prominent international journal Nature Chemical Biology, the research findings could allow the breeding of more efficient strains of oil-degrading bacteria.

Bonn researchers decode the interaction of monocytes and platelets in human blood

Monocytes, a special type of white blood cell, secrete cytokines as inflammatory messengers that are crucial for an appropriate immune response. Researchers at the University Hospital Bonn (UKB) and the University of Bonn have now discovered that platelets, also known as thrombocytes, communicate with monocytes and increase their inflammatory capacity. By understanding the platelet-monocyte interaction, they hope to improve the treatment of immune disorders and associated diseases. The results of the study have now been published in the renowned journal “EMBO Molecular Medicine” and will be featured on the cover of August issue.

Breakthrough in brown fat research

Researchers from the University of Southern Denmark, the Novo Nordisk Center for Adipocyte Signaling (SDU), the University of Bonn and the University Hospital Bonn (UKB) have found a protein that is responsible for turning off brown fat activity. This new discovery could lead to a promising strategy for safely activating brown fat and tackling obesity and related health problems. The results of the study have now been published in the journal „Nature Metabolism“.

Publications

2024

Abou Assale, Tawfik; Afrang, Negin; Wissfeld, Jannis; Cuevas-Rios, German; Klaus, Christine; Linnartz-Gerlach, Bettina; Neumann, Harald (2024): Neuroprotective role of sialic-acid-binding immunoglobulin-like lectin-11 in humanized transgenic mice. In: Frontiers in neuroscience 18, S. 1504765. DOI: 10.3389/fnins.2024.1504765.

Araki, Kunihiko; Schwering-Sohnrey, Merlin; Marku, Griselda; Pires, Nuno; Wenzel, Michael; Schoch, Susanne et al. (2024): Effects of dibenzazepine compounds on human Nav1.2 channels and neuronal network activity: A systematic comparison. In: Authorea Preprints. DOI: 10.22541/au.172708657.76144438/v1.

Becker, Katrin (2024): Ventricular lymphatic vessel density correlates with mild cardiac remodelling and preserved heart function in moderate murine aortic valve stenosis.

Cuevas-Rios, German; Assale, Tawfik Abou; Wissfeld, Jannis; Bungartz, Annemarie; Hofmann, Julia; Langmann, Thomas; Neumann, Harald (2024): Decreased sialylation elicits complement-related microglia response and bipolar cell loss in the mouse retina. In: Glia 72 (12), S. 2295–2312. DOI: 10.1002/glia.24613.

Fabiano, Marietta; Oikawa, Naoto; Kerksiek, Anja; Furukawa, Jun-ichi; Yagi, Hirokazu; Kato, Koichi et al. (2024): Presenilin Deficiency Results in Cellular Cholesterol Accumulation by Impairment of Protein Glycosylation and NPC1 Function. In: International Journal of Molecular Sciences 25 (10), S. 5417. DOI: 10.3390/ijms25105417.

Giordano, Frank A.; Layer, Julian P.; Leonardelli, Sonia; Friker, Lea L.; Turiello, Roberta; Corvino, Dillon et al. (2024): L-RNA aptamer-based CXCL12 inhibition combined with radiotherapy in newly-diagnosed glioblastoma: dose escalation of the phase I/II GLORIA trial. In: Nat Commun 15 (1), S. 4210. DOI: 10.1038/s41467-024-48416-9.

Hawwari, Ibrahim; Rossnagel, Lukas; Rosero, Nathalia; Maasewerd, Salie; Vasconcelos, Matilde B.; Jentzsch, Marius et al. (2024): Platelet transcription factors license the pro-inflammatory cytokine response of human monocytes. In: EMBO molecular medicine 16 (8), S. 1901–1929. DOI: 10.1038/s44321-024-00093-3.

Hoffmann, Alina; Steffens, Ursula; Maček, Boris; Franz-Wachtel, Mirita; Nieselt, Kay; Harbig, Theresa Anisja et al. (2024): The unusual mode of action of the polyketide glycoside antibiotic cervimycin C. In: mSphere 9 (5), e0076423. DOI: 10.1128/msphere.00764-23.

Islam, K. Ushna S.; Blaess, Sandra (2024): The impact of the mesoprefrontal dopaminergic system on the maturation of interneurons in the murine prefrontal cortex. In: Frontiers in neuroscience 18, S. 1403402. DOI: 10.3389/fnins.2024.1403402.

Jacob, Alice M.; Lindemann, Anna F.; Wagenpfeil, Julia; Geiger, Sergej; Layer, Yannik C.; Salam, Babak et al. (2024): Autofluorescence-based tissue characterization enhances clinical prospects of light-sheet-microscopy. In: Sci Rep 14 (1), S. 18033. DOI: 10.1038/s41598-024-67366-2.

Kapadia, Akshay; Theil, Sandra; Opitz, Sabine; Villacampa, Nàdia; Beckert, Hannes; Schoch, Susanne et al. (2024): Phosphorylation-state dependent intraneuronal sorting of Aβ differentially impairs autophagy and the endo-lysosomal system. In: Autophagy 20 (1), S. 166–187. DOI: 10.1080/15548627.2023.2252300.

Khani, Sajjad; Topel, Hande; Kardinal, Ronja; Tavanez, Ana Rita; Josephrajan, Ajeetha; Larsen, Bjørk Ditlev Marcher et al. (2024): Cold-induced expression of a truncated adenylyl cyclase 3 acts as rheostat to brown fat function. In: Nat Metab 6 (6), S. 1053–1075. DOI: 10.1038/s42255-024-01033-8.

Kim, Hyuntae; Melliti, Nesrine; Breithausen, Eva; Michel, Katrin; Colomer, Sara Ferrando; Poguzhelskaya, Ekaterina et al. (2024): Paroxysmal dystonia results from the loss of RIM4 in Purkinje cells. In: Brain : a journal of neurology 147 (9), S. 3171–3188. DOI: 10.1093/brain/awae081.

Kirchhoff, Alexander; Herzner, Anna-Maria; Urban, Christian; Piras, Antonio; Düster, Robert; Mahlberg, Julia et al. (2024): RNA-binding proteins hnRNPM and ELAVL1 promote type-I interferon induction downstream of the nucleic acid sensors cGAS and RIG-I. In: The EMBO Journal. DOI: 10.1038/s44318-024-00331-x.

Li, Yutao; Sharma, Amit; Hoffmann, Michèle J.; Skowasch, Dirk; Essler, Markus; Weiher, Hans; Schmidt-Wolf, Ingo G. H. (2024): Discovering single cannabidiol or synergistic antitumor effects of cannabidiol and cytokine-induced killer cells on non-small cell lung cancer cells. In: Frontiers in immunology 15, S. 1268652. DOI: 10.3389/fimmu.2024.1268652.

Luca, Domnica; Lee, Sumin; Hirota, Keiji; Okabe, Yasutaka; Uehori, Junji; Izawa, Kazushi et al. (2024): Aberrant RNA sensing in regulatory T cells causes systemic autoimmunity. In: Science Advances 10 (9), eadk0820. DOI: 10.1126/sciadv.adk0820.

Mangan, Matthew S. J.; Akbal, Anil; Rivara, Sophie; Schröder, Agnes; Horvath, Gabor; Walch, Philipp et al.: Cytosolic sodium accumulation is a common danger signal triggering endocytic dysfunction and NLRP3 inflammasome activation: bioRxiv. Online verfügbar unter https://www.biorxiv.org/content/10.1101/2024.03.26.586774v2, zuletzt geprüft am 22.11.2024.

Masala, Nicola; Mittag, Manuel; Ambrad Giovannetti, Eleonora; O’Neil, Darik A.; Distler, Fabian; Rupprecht, Peter et al. (2024): Aberrant hippocampal Ca2+ micro-waves following synapsin-dependent adenoviral expression of Ca2+ indicators.

Mitlasóczki, Bence; Gómez, Adrián Gutiérrez; Kamali, Midia; Babushkina, Natalia; Baues, Mayan; Kück, Laura et al. (2024): Region-specific spreading depolarization drives aberrant post-ictal behavior.

Nikbakht, Negar; Pofahl, Martin; Miguel-López, Albert; Kamali, Fateme; Tchumatchenko, Tatjana; Beck, Heinz (2024): Efficient encoding of aversive location by CA3 long-range projections. In: Cell Reports 43 (3), S. 113957. DOI: 10.1016/j.celrep.2024.113957.

Podoliak, E.; Lamm, G. H. U.; Marin, E.; Schellbach, A. V.; Fedotov, D. A.; Stetsenko, A. et al. (2024): A subgroup of light-driven sodium pumps with an additional Schiff base counterion. In: Nat Commun 15 (1), S. 3119. DOI: 10.1038/s41467-024-47469-0.

Schiffelers, Lisa D. J.; Tesfamariam, Yonas M.; Jenster, Lea-Marie; Diehl, Stefan; Binder, Sophie C.; Normann, Sabine et al. (2024): Antagonistic nanobodies implicate mechanism of GSDMD pore formation and potential therapeutic application. In: Nat Commun 15 (1), S. 8266. DOI: 10.1038/s41467-024-52110-1.

Schmidt, Elena N. C.; Evert, Bernd O.; Pregler, Barbara E. F.; Melhem, Ahmad; Hsieh, Meng-Chun; Raspe, Markus et al. (2024): Tonabersat enhances temozolomide-mediated cytotoxicity in glioblastoma by disrupting intercellular connectivity through connexin 43 inhibition. In: Molecular Oncology. DOI: 10.1002/1878-0261.13786.

Sieckmann, Katharina; Winnerling, Nora; Silva Ribeiro, Dalila Juliana; Kardinal, Ronja; Steinheuer, Lisa Maria; Schermann, Geza et al. (2024): BBSome-dependent ciliary Hedgehog signaling governs cell fate in the white adipose tissue.

Tan, Rui Lois; Sciandra, Francesca; Hübner, Wolfgang; Bozzi, Manuela; Reimann, Jens; Schoch, Susanne et al. (2024): The missense mutation C667F in murine β-dystroglycan causes embryonic lethality, myopathy and blood-brain barrier destabilization. In: Disease Models & Mechanisms 17 (6). DOI: 10.1242/dmm.050594.

Theodorou, Maria Rafailia; Yu, Jiangyan; Nikolka, Fabian; Zurkovic, Jelena; Wientjens, Chantal; Weiss, Patricia et al.: Anabolic lipid metabolism regulates adipose type 2 innate lymphoid cell differentiation to maintain metabolic health: bioRxiv. Online verfügbar unter https://www.biorxiv.org/content/10.1101/2024.03.26.586766v1, zuletzt geprüft am 22.11.2024.

van Baarle, Lies; Simone, Veronica de; Schneider, Linda; Santhosh, Sneha; Abdurahiman, Saeed; Biscu, Francesca et al. (2024): IL-1R signaling drives enteric glia-macrophage interactions in colorectal cancer. In: Nat Commun 15 (1), S. 6079. DOI: 10.1038/s41467-024-50438-2.

Weiten, Richard; Bernhardt, Marit; Niemann, Max; Kristiansen, Glen; Grünwald, Viktor; Ritter, Manuel et al. (2024): Exploring membranous NECTIN-4 expression patterns and enfortumab vedotin response in prostate cancer. In: Journal of cellular and molecular medicine 28 (14), e18572. DOI: 10.1111/jcmm.18572.

Weiten, Richard; Niemann, Max; Below, Eduard; Friker, Lea L.; Ralser, Damian J.; Toma, Marieta et al. (2024): Preclinical evidence for the use of anti-Trop-2 antibody-drug conjugate Sacituzumab govitecan in cerebral metastasized castration-resistant prostate cancer. In: Cancer medicine 13 (12), e7320. DOI: 10.1002/cam4.7320.

2023

Antoniou, Anna; Auderset, Loic; Kaurani, Lalit; Sebastian, Eva; Zeng, Yuzhou; Allahham, Maria et al. (2023): Neuronal extracellular vesicles and associated microRNAs induce circuit connectivity downstream BDNF. In: Cell Reports 42 (2), S. 112063. DOI: 10.1016/j.celrep.2023.112063.

Jenster, Lea-Marie; Lange, Karl-Elmar; Normann, Sabine; vom Hemdt, Anja; Wuerth, Jennifer D.; Schiffelers, Lisa D. J. et al. (2023): P38 kinases mediate NLRP1 inflammasome activation after ribotoxic stress response and virus infection. In: The Journal of experimental medicine 220 (1). DOI: 10.1084/jem.20220837.

Leven, Patrick; Schneider, Reiner; Schneider, Linda; Mallesh, Shilpashree; Vanden Berghe, Pieter; Sasse, Philipp et al. (2023): β-adrenergic signaling triggers enteric glial reactivity and acute enteric gliosis during surgery. In: Journal of neuroinflammation 20 (1), S. 255. DOI: 10.1186/s12974-023-02937-0.

Merges, Gina E.; Arévalo, Lena; Kovacevic, Andjela; Lohanadan, Keerthika; Rooij, Dirk G. de; Simon, Carla et al. (2023): Actl7b deficiency leads to mislocalization of LC8 type dynein light chains and disruption of murine spermatogenesis. In: Development (Cambridge, England) 150 (21). DOI: 10.1242/dev.201593.

Niepmann, Sven Thomas; Willemsen, Nicola; Boucher, Ann Sophie; Stei, Marta; Goody, Philip; Zietzer, Andreas et al. (2023): Toll-like receptor-3 contributes to the development of aortic valve stenosis. In: Basic research in cardiology 118 (1), S. 6. DOI: 10.1007/s00395-023-00980-9.

Próchnicki, Tomasz; Vasconcelos, Matilde B.; Robinson, Kim S.; Mangan, Matthew S. J.; Graaf, Dennis de; Shkarina, Kateryna et al. (2023): Mitochondrial damage activates the NLRP10 inflammasome. In: Nat Immunol 24 (4), S. 595–603. DOI: 10.1038/s41590-023-01451-y.

Schneider, Simon; Kovacevic, Andjela; Mayer, Michelle; Dicke, Ann-Kristin; Arévalo, Lena; Koser, Sophie A. et al. (2023): Cylicins are a structural component of the sperm calyx being indispensable for male fertility in mice and human. In: eLife 12. DOI: 10.7554/eLife.86100.

Tsukamoto, Yuta; Hiono, Takahiro; Yamada, Shintaro; Matsuno, Keita; Faist, Aileen; Claff, Tobias et al. (2023): Inhibition of cellular RNA methyltransferase abrogates influenza virus capping and replication. In: Science (New York, N.Y.) 379 (6632), S. 586–591. DOI: 10.1126/science.add0875.

2022

Arévalo, Lena; Merges, Gina Esther; Schneider, Simon; Oben, Franka Enow; Neumann, Isabelle Sophie; Schorle, Hubert (2022): Loss of the cleaved-protamine 2 domain leads to incomplete histone-to-protamine exchange and infertility in mice. In: PLoS Genet 18 (6), e1010272. DOI: 10.1371/journal.pgen.1010272.

Bertheloot, Damien; Wanderley, Carlos Ws; Schneider, Ayda H.; Schiffelers, Lisa Dj; Wuerth, Jennifer D.; Tödtmann, Jan Mp et al. (2022): Nanobodies dismantle post-pyroptotic ASC specks and counteract inflammation in vivo. In: EMBO molecular medicine 14 (6), e15415. DOI: 10.15252/emmm.202115415.

Dietrich, Alina; Steffens, Ursula; Gajdiss, Mike; Boschert, Anna-Lena; Dröge, Jana Katharina; Szekat, Christiane et al. (2022): Cervimycin-Resistant Staphylococcus aureus Strains Display Vancomycin-Intermediate Resistant Phenotypes. In: Microbiology Spectrum 10 (5), e0256722. DOI: 10.1128/spectrum.02567-22.

Fouda, Ahmed Mahmoud; Atta, Osama; Kassem, Amr Shebl; Desoky, Mohamed; Bourauel, Christoph (2022): Wear behavior and abrasiveness of monolithic CAD/CAM ceramics after simulated mastication. In: Clinical Oral Investigations 26 (11), S. 6593–6605. DOI: 10.1007/s00784-022-04611-w.

Garofano, Francesca; Sharma, Amit; Abken, Hinrich; Gonzalez-Carmona, Maria A.; Schmidt-Wolf, Ingo G. H. (2022): A Low Dose of Pure Cannabidiol Is Sufficient to Stimulate the Cytotoxic Function of CIK Cells without Exerting the Downstream Mediators in Pancreatic Cancer Cells. In: International Journal of Molecular Sciences 23 (7). DOI: 10.3390/ijms23073783.

Hansen, Jan N.; Kaiser, Fabian; Leyendecker, Philipp; Stüven, Birthe; Krause, Jens-Henning; Derakhshandeh, Fatemeh et al. (2022): A cAMP signalosome in primary cilia drives gene expression and kidney cyst formation. In: EMBO Reports 23 (8), e54315. DOI: 10.15252/embr.202154315.

Häusl, Alexander S.; Bajaj, Thomas; Brix, Lea M.; Pöhlmann, Max L.; Hafner, Kathrin; Angelis, Meri de et al. (2022): Mediobasal hypothalamic FKBP51 acts as a molecular switch linking autophagy to whole-body metabolism. In: Science Advances 8 (10), eabi4797. DOI: 10.1126/sciadv.abi4797.

Leven, Patrick; Schneider, Reiner; Siemens, Kevin D.; Jackson, Walker S.; Wehner, Sven (2022): Application of a RiboTag-based approach to generate and analyze mRNA from enteric neural cells. In: Neurogastroenterology and motility 34 (7), e14309. DOI: 10.1111/nmo.14309.

Merges, Gina Esther; Meier, Julia; Schneider, Simon; Kruse, Alexander; Fröbius, Andreas Christian; Kirfel, Gregor et al. (2022): Loss of Prm1 leads to defective chromatin protamination, impaired PRM2 processing, reduced sperm motility and subfertility in male mice. In: Development (Cambridge, England) 149 (12). DOI: 10.1242/dev.200330.

Müller, Johannes Alexander; Betzin, Julia; Santos-Tejedor, Jorge; Mayer, Annika; Oprişoreanu, Ana-Maria; Engholm-Keller, Kasper et al. (2022): A presynaptic phosphosignaling hub for lasting homeostatic plasticity. In: Cell Reports 39 (3), S. 110696. DOI: 10.1016/j.celrep.2022.110696.

Royero, Pedro; Quatraccioni, Anne; Früngel, Rieke; Silva, Mariella Hurtado; Bast, Arco; Ulas, Thomas et al. (2022): Circuit-selective cell-autonomous regulation of inhibition in pyramidal neurons by Ste20-like kinase. In: Cell Reports 41 (10), S. 111757. DOI: 10.1016/j.celrep.2022.111757.

Sieckmann, Katharina; Winnerling, Nora; Huebecker, Mylene; Leyendecker, Philipp; Juliana Silva Ribeiro, Dalila; Gnad, Thorsten et al. (2022): AdipoQ-a simple, open-source software to quantify adipocyte morphology and function in tissues and in vitro. In: Molecular Biology of the Cell 33 (12), br22. DOI: 10.1091/mbc.E21-11-0592.

Singh, Jasjot; Elhabashy, Hadeer; Muthukottiappan, Pathma; Stepath, Markus; Eisenacher, Martin; Kohlbacher, Oliver et al. (2022): Cross-linking of the endolysosomal system reveals potential flotillin structures and cargo. In: Nat Commun 13 (1), S. 6212. DOI: 10.1038/s41467-022-33951-0.