Equipment
The Core Facility Translational Proteomics specializes in processing proteomic samples in a clinical context using high-throughput methods. Liquid handling systems prepare a variety of sample types, including cell and tissue lysates as well as biofluids, for proteomic measurements. State-of-the-art liquid chromatography mass spectrometry systems provide highly sensitive and precise proteomics data, enhancing scientific projects with high-quality results.
Liquid Chromatography - Mass spectrometry
Thermo Orbitrap Astral high-resolution MS
High-speed, high-sensitivity hybrid mass spectrometer with a novel design mass analyzer
The Orbitrap Astral mass spectrometer combines well-established mass analyzers (quadrupole and Orbitrap analyzer) with a completely new time-of-flight-like analyzer type: the Asymmetric Track Lossless (Astral) analyzer. While the Orbitrap analyzer is known for high dynamic range and high-resolution acquisition, the Astral analyzer excels in scan speed and sensitivity. When operated in data-independent acquisition (DIA) mode, the system achieves a scan speed of 200 Hz at a resolution of 80,000 and provides single ion detection sensitivity. Synchronized operation among the three analyzers enables a nearly 100% duty cycle.
Thermo Vanquish Neo ultrahigh-performance liquid chromatography
The Thermo Vanquish Neo liquid chromatography system is suitable for high throughput, reproducibility, and sensitivity and therefore the system of choice for clinical samples. Direct injection or the utilization of trap columns, as well as the broad range of flow rates, including nanoliter-, capillary-, and microliter flow rates, make the system very versatile and suitable for all of our offered workflows.
Bruker timsTOF Pro 2 high-resolution MS
High-speed, high-sensitivity hybrid mass spectrometer with an additional ion separation dimension
The timsTOF Pro 2 mass spectrometer separates ion species by their mass-to-charge ratio in the quadrupole and their collisional cross sections using a dual Trapped Ion Mobility Spectrometry (TIMS) analyzer. By Parallel Accumulation Serial Fragmentation (PASEF®), the timsTOF Pro 2 offers an additional dimension for peptide separation and reaches a close to 100% duty cycle at a scan speed of >120 Hz. The fast and ultrasensitive time-of-flight (TOF) analyzer provides high-resolution results at near single-ion detection sensitivity.
Thermo Stellar mass spectrometer
High-speed, high-sensitivity mass spectrometer for targeted quantitation of thousands of molecules in a single run
The Stellar mass spectrometer combines robust triple-quadrupole technology with an ultrafast and sensitive dual-pressure linear ion trap analyzer. Its strength is the targeted quantitation of thousands of molecules, for example, peptide species. Software specifically developed for the Stellar MS facilitates the automated transfer of results from discovery measurements into targeted methods.
Evosep One ultrahigh-performance liquid chromatography
The Evosep One liquid chromatography system is suitable for high throughput, reproducibility, and sensitivity and, therefore, the system of choice for clinical samples. Standardized sample preparation, automated sample loading, and predefined chromatographic gradients are the unique features of this system.
Automated Liquid Handling
Agilent AssayMap Bravo
Robust and reproducible liquid handling system for high throughput digestion
The Agilent AssayMap Bravo is a liquid handling system optimized for the high throughput processing of proteomics samples. Predesigned protocols for protein in-solution and protein aggregation capture (PAC) digestion, as well as procedures for immunoprecipitation and enrichment of posttranslationally modified peptides, e.g., phosphopeptides, allow streamlined processing of proteomics samples. The system is highly robust and reproducible and keeps batch effects as small as possible, a prerequisite for clinical proteomics studies.
Hamilton SP100
Fully automated processing of plasma samples for deep plasma proteomics
The Hamilton SP100 is used for the automated sample preparation of liquid biopsies such as blood plasma for deep plasma proteomics. It uses the Seer Proteograph XT workflow and is the prerequisite for measuring deep plasma proteomes by mass spectrometry.
Opentrons OT-2
High-precision and versatile open-source liquid handling system
The Opentrons OT-2 is a versatile and modular liquid handling system applicable to many different workflows. By its open-source, python-based format, thousands of community-based protocols are available.
Address
Translational Proteomics
Please contact us upon arrival at the foyer and we will pick you up.
How to find us
Arrival by bus and train or map
From Bonn main station you can take the bus line 600 (direction Ippendorf Altenheim) and 601 (direction Venusberg) to the bus stop “Uniklinikum Nord, Uniklinikum Hauptpforte or Uniklinikum Süd”. Line 630 runs in the Bonn city area without stopping at the main station. Please use the night bus (N5) between midnight and 5:30 a.m.
The Core Facilities thank the German Research Foundation for continuous support.
