Frequently Asked Questions

A: We can offer you our expertise and support for your project, which includes experimental design tailored to support your goals. You do not need to have a deep understanding of proteomics beforehand, as we will guide you through the process and provide you with the necessary knowledge.

A: The required amount of sample for proteomic analyses depends on the sample preparation approach and the type of analysis planned. For a label-free quantitative proteomics experiment, a minimum of 5 µg of total protein per sample is recommended. We do offer protocols for smaller sample amounts but it may result in a reduced number of protein identifications. We suggest a minimum of 100 µg total protein per sample for a phosphoproteomic analysis. While smaller input is possible as well, it may lead to a decreased identification of phosphopeptides.

A: The approach for preparing and handling samples depends on the type of sample and the research question. It is important to note that we only accept projects that have been discussed with us in advance. This ensures that we can provide you with the appropriate guidance on how to optimally prepare, store, and transfer your samples.

A: To ensure that your samples are processed accurately, we require you to fill out a sample submission sheet. Once we have discussed your project, we will provide you with the sheet or you can directly download it from our website. After filling it out, kindly send it back to us. The information provided on the submission sheet enables us to track your samples, select the best sample processing workflow, and carry out subsequent analysis.

A: The processing and analysis of samples at the Core Facility depend on the availability of instruments and the current sample load. Samples are handled on a first-come, first-served basis. For a standard project, the workflow from submission to evaluated data usually takes around 4 to 6 weeks.

A: We store all acquired raw data and evaluation results on our servers for a minimum of 1 year. A backup of your data for long-term storage is planned.