Zeiss CellDiscoverer 7
High-content screen widefield fluorescence microscope for automated imaging of several multi-well plates, equipped with plate loader robotics. The incubation chamber allows for temperature and CO2 control to provide optimal conditions for live cell imaging.
Funding information:
Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) – Projektnummer 388158066 (see further information for acknowledgment)
Objectives:
| Magnification | Type | NA | Immersion |
| 5x | PlanApochromat, LD | 0.35 | dry |
| 20x | PlanApochromat, LD | 0.70 autocorr | dry |
| 20x | PlanApochromat | 0.95 autocorr | dry |
| 50x | PlanApochromat | 1.20 autocorr | water |
Afocal magnification changer (0.5X / 1.0X / 2.0X) is available for all objectives.
The 50X objective is equipped with automated water dispenser.
Optical setup:
Light sources:
LED Set 01: 385, 470, 567 and 625 nm
LED Set 02: 420, 511 and 590 nm
Dichroic mirrors:
Quad-band (Zeiss, set 90 HE)
Tri-band (Zeiss, set 91 HE)
Emission filters:
Quad-band (Zeiss, set 90 HE)
Tri-band (Zeiss, set 91 HE)
CFP, 465/30 (AHF, F39-465)
GFP, 524/24 (AHF, F39-527)
YFP, 555/25 (AHF, F49-555)
RFP, 590/20 (AHF, F37-592)
The multi-band setup:
| Color | BFP | CFP | GFP | YFP | RFP | AF594 | AF647 |
| LED and filter set | 1 | 2 | 1 | 2 | 1 | 2 | 1 |
| Excitation | 385 | 420 | 470 | 511 | 567 | 590 | 625 |
| Dichroic | 405 | 450 | 493 | 538 | 575 | 610 | 653 |
| Emission | 425/30 | 467/24 | 514/30 | 555/25 | 592/25 | 687/145 | 709/100 |
Cameras:
Axiocam 506m
Accessories:
Definite Focus.2 for stabilized z-focus
CO2 and temperature incubator system
Plate loader with 2x12 plate carousel
Software:
ZEN Blue 2.6 Pro
Modules: Tiles&Positions, Autofocus, Experimental Designer, Image Analysis, Advanced Processing, Measurement, Physiological package, Macro Environment
Funding
Deutsche Forschungsgemainschaft (DFG, German Research Foundation) – Projektnummer 388158066
The Core Facilities thank the German Research Foundation for continuous support.
