Leica SP8 Lightning
Point-scanning confocal microscope for imaging fixed and live samples with high resolution. The Lightning deconvolution modules enables enhanced resolution. The incubation chamber allows for temperature and CO2 control to provide optimal conditions for live cell imaging.
Funding information:
Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) – Projektnummer 388159768 (see further information for acknowledgment)
Objectives:
| Magnification | Type | NA | Immersion |
| 10x | HC PL Fluotar | 0.30 | dry |
| 20x | HC PL APO CS2 | 0.75 corr | multi |
| 40x | HC PL APO CS2 | 1.10 corr | water |
| 63x | HC PL APO CS2 | 1.20 corr | water |
| 63x | HC PL APO CS2 | 1.40 | oil |
Lasers:
Diode (405 nm)
Argon, multiline (458, 476, 488, 496 and 514 nm)
DPSS (561 nm)
HeNe (633 nm)
Accessories:
4 x HyD detectors for the best signal-to-noise ratio
Resonance scanner (8 kHz) for fast live imaging with minimal photo-toxicity
CO2 and temperature incubator system
Software:
LAS X v3.7.1
Wizards: FRAP, FRET, Lightning, Live Data Mode, Dye Finder, 3D Visualization, Navigator
Lightning deconvolution software
The Lightning module automatically detects the finest structures and details. The key is an adaptive process for extraction of hidden information in the image. Unlike traditional deconvolution techniques, that use a global set of parameters for the full image, Lightning calculates an appropriate set of parameters for each voxel to uncover every detail with the highest fidelity.
The Lightning detection concept extends the confocal microscope by simultaneous multi-color super-resolution imaging at the full original speed.
Funding
Deutsche Forschungsgemainschaft (DFG, German Research Foundation) – Projektnummer 388159768
The Core Facilities thank the German Research Foundation for continuous support.
