Olympus IX81

The Olympus IX81 is a motorized inverted fluorescence microscope. It supports transmitted light and fluorescence imaging with multiple filter configurations, allowing observation of fixed specimens. With the attached Abberrior STEDYCON module, also superresolution imaging is possible. The motorized stage and focus control enable automated image acquisition for multi-position experiments. Although an older model, the IX81 remains a dependable system for everyday microscopy tasks.

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Objectives:

Mag. Type NA Immersion WD (mm) Coverslip (µm)
2.5x Plan NEOFLUAR (Zeiss) 0.075 dry -
4x Plan N 0.1 dry 18.5 -
20x LCPlanFl Ph1 0.4 dry 3 (LWD) 0.17
40x UPlanApo 0.85 dry 0.2 0.11 - 0.23 (corr)
60x PlanApo 1.4 oil 0.15 0.17
100x UPlanSApo 1.4 oil 0.13 0.17

Light Source:

Olympus MT20 fluorescence lamp

Filters:

Excitation: 387/11nm, 485/20nm, 560/25nm, 650/13nm

Dichroic mirrors: 410/504/582/669

Emission: 440/40nm, 525/30nm, 607/36nm, 685/24nm

Camera:

Hamamatsu Orca Flash 4.0 LT+ (monochrome camera)

Additional modules:

Abberior STEDYCON (STED module)

Software:

Olympus software


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