Olympus IX81
The Olympus IX81 is a motorized inverted fluorescence microscope. It supports transmitted light and fluorescence imaging with multiple filter configurations, allowing observation of fixed specimens. With the attached Abberrior STEDYCON module, also superresolution imaging is possible. The motorized stage and focus control enable automated image acquisition for multi-position experiments. Although an older model, the IX81 remains a dependable system for everyday microscopy tasks.
Objectives:
| Mag. | Type | NA | Immersion | WD (mm) | Coverslip (µm) |
| 2.5x | Plan NEOFLUAR (Zeiss) | 0.075 | dry | - | |
| 4x | Plan N | 0.1 | dry | 18.5 | - |
| 20x | LCPlanFl Ph1 | 0.4 | dry | 3 (LWD) | 0.17 |
| 40x | UPlanApo | 0.85 | dry | 0.2 | 0.11 - 0.23 (corr) |
| 60x | PlanApo | 1.4 | oil | 0.15 | 0.17 |
| 100x | UPlanSApo | 1.4 | oil | 0.13 | 0.17 |
Light Source:
Olympus MT20 fluorescence lamp
Filters:
Excitation: 387/11nm, 485/20nm, 560/25nm, 650/13nm
Dichroic mirrors: 410/504/582/669
Emission: 440/40nm, 525/30nm, 607/36nm, 685/24nm
Camera:
Hamamatsu Orca Flash 4.0 LT+ (monochrome camera)
Additional modules:
Abberior STEDYCON (STED module)
Software:
Olympus software
The Core Facilities thank the German Research Foundation for continuous support.