Visitron Visiscope
The Visitron VisiScope system with Homogenizer is a high-end fluorescence imaging platform optimized for live-cell imaging and larger volume acquisitions. Integrated with a homogenizer for uniform illumination across the field of view, the system ensures consistent excitation intensity. The microscope supports widefield and spinning disk confocal imaging with sensitive sCMOS cameras at high speed.
Funding information:
Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) – Projektnummer 388169927 (see further information for acknowledgment)
Objectives:
| Mag. | Type | NA | Immersion | WD (mm) | Coverslip (µm) |
| 5x | PlanNeofluar | 0.16 | dry | 18.5 | 0.17 |
| 10x | PlanApochromat | 0.45 | dry | 2 | 0.17 |
| 20x | PlanApochromat | 0.8 | dry | 0.55 | 0.17 |
| 40x | C-Apochromat | 1.2 | water | ~ 0.28 | 0.14 - 0.19 |
| 63x | C-Apochromat | 1.2 | water | ~ 0.28 | 0.14 - 0.19 |
| 63x | C-Apochromat | 1.4 | oil | 0.14 | 0.17 |
Spinning Disk unit:
Yokogawa CSU-W1 with 25 µm and 50 µm pinhole disk
Laser lines:
405 nm, 488 nm, 561 nm, 640 nm
Filters:
DAPI, GFP, mCherry, Cy5
Cameras:
Two pco.edge sCMOS cameras
Accessories:
VS-Homogenizer for a flat intensity profile in the field of view
Definite Focus.2 for stabilized z-focus
CO2 (0-15%) and temperature (25-60°C) incubator system
Automated water immersion dispenser (for 40x and 63x water)
Piezo z-stage
Microscope stand:
Zeiss Axio Observer
Software:
VisiView Software
Funding
Deutsche Forschungsgemainschaft (DFG, German Research Foundation) – Projektnummer 388169927
The Core Facilities thank the German Research Foundation for continuous support.
