Zeiss Observer.Z1
Widefield fluorescence microscope for live cell imaging and fixed samples. Dual cam supports acquisition of two channels in parallel. The incubation chamber allows for temperature and CO2 control to provide optimal conditions for live cell imaging.
Funding information:
Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) – Projektnummer 13123509 (SFB 670) (see further information for acknowledgment)
Objectives:
| Mag. | Type | NA | Immersion | Ph | DIC |
| 2.5x | LD N-AchroPlan | 0.07 | dry | ||
| 10x | LD EC PlanNeoFluor | 0.30 | dry | 1 | II |
| 20x | LD PlanNeoFluor | 0.40 corr | dry | II | |
| 10x | PlanApochromat | 0.45 | dry | II | |
| 20x | PlanApochromat | 0.80 | dry | ||
| 40x | PlanApochromat | 0.95 corr | dry | II | |
| 40x | PlanApochromat | 1.40 | oil |
Excitation source:
HXP lamp, Colibri 1
Filters:
BFP (AHF)
Sapphire (AHF)
DuoLink dual camera sets:
GFP / mCherry (Zeiss, set 79 HE)
GEM-GECO (AHF)
Cameras:
DuoLink dual camera adapter
2 x Axiocam 506m
1 x Axiocam 506c
Accessories:
ApoTome.2 for optical sectioning
Definite Focus for stabilized z-focus
CO2 and temperature incubator system
Software:
ZEN Blue 2.6 Pro
Physiological package
Funding
Deutsche Forschungsgemainschaft (DFG, German Research Foundation) – Projektnummer 13123509 (SFB 670)
The Core Facilities thank the German Research Foundation for continuous support.
