Scope and Expertise
Types of Analyses
- Identification and characterization of proteins
- Detection of posttranslational modifications
- Comparative quantitative analyses of complex proteomes.
We work in close collaboration with the Core Facility Translational Proteomics in order to share expertise, protocols, and ressources. If you have >100 human samples in your project you can get in touch with them directly. Our common aim is to offer the best possible proteomics services to the scientific community in Bonn and beyond.
Methods we apply
We use ESI-LC/MS instruments to measure intact protein masses, identify proteins after proteolytic digestion (from polyacrylamide gels or protein solutions) or analyze post translational modifications. Our Orbitrap mass spectrometers with high resolution and sensitivity allow analyses of complex samples such as cell lysates with or without isotope labeling (SILAC, isobaric TMT). Please look at our instruments page for more details.
Data analysis
The data we provide can be very informative but also challenging, in particular the quantitative characterization of proteomes. Therefore, early consultation with us is essential to improve the chances for a successful mass spectrometric analysis. In particular we should discuss the strategy, scope, and pitfalls of these experiments as well as the sample characteristics.
We collaborate closely with the Core Unit Bioinformatics for design, characterization, visualization, and interpretation of complex data sets. We will support you in utilizing your data to develop meaningful working hypotheses and gain biological insights. Please refer to our publications section for examples.
The Core Facilities thank the German Research Foundation for continuous support.
